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t7 bioarray high yield rna transcript labeling kit  (Enzo Biochem)

 
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    Structured Review

    Enzo Biochem t7 bioarray high yield rna transcript labeling kit
    T7 Bioarray High Yield Rna Transcript Labeling Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high-yield+rna+transcript+labeling+kit+(t7/bioarray+high+yield+rna+transcript+labelling+kit/pmc01513230-163-5-13
    Average 90 stars, based on 1 article reviews
    t7 bioarray high yield rna transcript labeling kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Sexually dimorphic gene expression that overlaps maturation of type II pneumonocytes in fetal mouse lungs
    Article Snippet: .. For the second amplification, a T7 BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY) was used to produce biotinylated cRNA. .. The mixture (20 μl final volume) was incubated at 37°C for 5 h with gentle mixing every 30 min. Labelled cRNA was purified using a RNeasy Mini Kit (Qiagen) according to the protocol of the manufacturer.

    Labeling:

    Article Title: Sexually dimorphic gene expression that overlaps maturation of type II pneumonocytes in fetal mouse lungs
    Article Snippet: .. For the second amplification, a T7 BioArray High Yield RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY) was used to produce biotinylated cRNA. .. The mixture (20 μl final volume) was incubated at 37°C for 5 h with gentle mixing every 30 min. Labelled cRNA was purified using a RNeasy Mini Kit (Qiagen) according to the protocol of the manufacturer.

    Article Title: Distinct patterns of gene and protein expression elicited by organophosphorus pesticides in Caenorhabditis elegans
    Article Snippet: Two micrograms of poly(A)+ RNA (adjusted for rRNA contamination) was used as the template for cDNA synthesis using the SuperScript Choice Kit (Invitrogen) per the manufacturer's recommendations except that (1) a high pressure liquid chromatography (HPLC)-purified T 24 T7 promoter primer (Integrated DNA Technologies, Coralville, IA) was used to initiate first strand synthesis; (2) the second strand synthesis was not terminated using EDTA since we found that EDTA carryover interfered with subsequent enzymatic manipulations; and (3) PelletPaint (Novagen, Madison, WI) was used in place of glycogen for precipitation. .. Biotin labeled cRNA was synthesized from the T7 promoter incorporated in the cDNA using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences, Farmingdale, NY) per the manufacturer's recommendations; approximately 1 μg of cDNA was used for synthesis. .. cRNA was purified from unincorporated nucleotides and other reaction components using the RNeasy Mini Kit (Qiagen).

    Synthesized:

    Article Title: Distinct patterns of gene and protein expression elicited by organophosphorus pesticides in Caenorhabditis elegans
    Article Snippet: Two micrograms of poly(A)+ RNA (adjusted for rRNA contamination) was used as the template for cDNA synthesis using the SuperScript Choice Kit (Invitrogen) per the manufacturer's recommendations except that (1) a high pressure liquid chromatography (HPLC)-purified T 24 T7 promoter primer (Integrated DNA Technologies, Coralville, IA) was used to initiate first strand synthesis; (2) the second strand synthesis was not terminated using EDTA since we found that EDTA carryover interfered with subsequent enzymatic manipulations; and (3) PelletPaint (Novagen, Madison, WI) was used in place of glycogen for precipitation. .. Biotin labeled cRNA was synthesized from the T7 promoter incorporated in the cDNA using the BioArray High Yield RNA Transcript Labeling Kit (Enzo Life Sciences, Farmingdale, NY) per the manufacturer's recommendations; approximately 1 μg of cDNA was used for synthesis. .. cRNA was purified from unincorporated nucleotides and other reaction components using the RNeasy Mini Kit (Qiagen).

    other:

    Article Title: Porcine Neural Progenitor Cells Derived from Tissue at Different Gestational Ages Can Be Distinguished by Global Transcriptome
    Article Snippet: A part of the resulting double-stranded cDNA was used as a template to generate biotin-tagged complementary RNA (cRNA) from an in vitro transcription reaction, using the Bioarray High Yield ® RNA transcript labeling kit (T7; Enzo Diagnostics, Inc., Farmingdale, NY, USA).

    Article Title: A Functional, Genome-wide Evaluation of Liposensitive Yeast Identifies the “RE2 Required for Viability” (ARV1) Gene Product as a Major Component of Eukaryotic Fatty Acid Resistance
    Article Snippet: RNA prepared as above from indicated mice was used for double strand cDNA synthesis and oligonucleotide array hybridization using SuperScript Choice System for cDNA synthesis (Invitrogen) with GeneChip T7-Oligo(dT) promoter primers (Affymetrix, Santa Clara, CA). cRNA were synthesized and labeled with biotin using a BioArray high yield RNA transcript labeling kit (T7) (Enzo, Farmingdale, NY).

    Article Title: Runx1 Loss Minimally Impacts Long-Term Hematopoietic Stem Cells
    Article Snippet: 450 ng of cDNA was then used as template for a second in vitro transcription in which biotin-labeled aRNA was generated with the BioArray High Yield RNA Transcript labeling kit (T7) (Enzo Life Sciences, Plymouth Meeting PA) according to the manufacturer's instructions.

    Article Title: Nkx1-2 is a transcriptional repressor and is essential for the activation of Brachyury in P19 mouse embryonal carcinoma cell
    Article Snippet: Total RNA was extracted from P19 cells that were treated with 2 μM BIO or vehicle control (0.02% DMSO) for 24 h, using TRI reagent (Molecular Research Center, Cincinnati, OH). cDNA probes were synthesized, using Genechip Expression 3′ Amplification One-Cycle cDNA Synthesis kit (Affymetrix, Santa Clara, CA) and Bioarray High Yield RNA Transcript Labeling Kit (T7) (Enzo Life Sciences, Farmingdale, NY), and were hybridized to mouse genome 430 A 2.0 Array (Affymetrix), according to the manufacturer’s protocol.

    Article Title: A Functional, Genome-wide Evaluation of Liposensitive Yeast Identifies the “ A RE2 R equired for V iability” ( ARV1 ) Gene Product as a Major Component of Eukaryotic Fatty Acid Resistance
    Article Snippet: Membranes were hybridized with PCR-generated hARV1 probe (Stratagene) and analyzed by phosphorimaging (GE Healthcare).

    Article Title: Human trophoblast responses to Porphyromonas gingivalis infection
    Article Snippet: In vitro transcription was performed using a BioArray high-yield RNA transcript labeling kit (T7) (Enzo Life Science, Farmingdale, NY), to incorporate biotinylated nucleotides.



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